Session Index

Biophotonics and Biomedical Imaging

Imaging & Microscopy II
Friday, Dec. 8, 2017  08:30-10:15
Presider: Yi-Chun Chen;Chia-Wei Sun
Room: International Research Building IR5008(5F)
08:30 - 08:45 Paper No.  2017-FRI-S0603-O001
Sung-Liang Chen Miniature Scan Head for All-Optical Optical-Resolution Photoacoustic Microscopy
Zhendong Guo;Guangyao Li;Sung-Liang Chen

Optical-resolution photoacoustic microscopy (OR-PAM) provides high lateral resolution and has demonstrated broad biomedical applications. We demonstrate a miniature scan head for all-optical OR-PAM using gradient-index lenses and a Fabry-Perot optical ultrasound detector. The scan head is easy to build and can be further developed for endoscopic and intravascular applications.

08:45 - 09:00 Paper No.  2017-FRI-S0603-O002
Ting-Wei Hsu Spectroscopic Full-Field Optical Coherence Tomography on In Vivo Rat Cornea Analysis
Ting-Wei Hsu;Yu-Tung Chen;Sheng-Lung Huang

Spectroscopic full-field optical coherence tomography (S-FFOCT) for in-vivo biomedical samples is demonstrated. Such optical system enabled high speed tomographic imaging as well as the spectroscopic analysis of the scanned data. Depth-dependent spectral peak shifts are observed in in-vivo rat cornea analysis.

09:00 - 09:15 Paper No.  2017-FRI-S0603-O003
Jye-Chang Lee The identification of tumor margin for extramammary Paget’s disease with harmonic generation microscopy
Jye-Chang Lee;Yi-Hua Liao;Chi-Kuang Sun

Identifying the tumor border of extramammary Paget’s disease (EMPD) by visual inspection is not accurate. Although skin biopsy provides a gold-standard of diagnosis and surgical margins, it’s invasive and time-consuming. Here, we presented the applications of HGM on determining the margins of EMPD, which is consistent with the histopathological results.

09:15 - 09:30 Paper No.  2017-FRI-S0603-O004
Feng Chun Hsu Volumetric Bioimaging Based on Light Field Microscopy with Temporal Focusing Illumination
Feng Chun Hsu;Yong Da Sie;Chia Yuan Chang;Feng Jie Lai;Shean Jen Chen

Light field technique at a single shot can get the whole volume image of observed sample. By integrating a light field microscopy to a temporal focusing-based multiphoton excitation fluorescence microscope, the excitation volume can be controlled. Therefore, light field microscope based on temporal focusing obtains three-dimensional image with less noise.

09:30 - 09:45 Paper No.  2017-FRI-S0603-O005
Sandeep Chakraborty Label-free Second and Third Harmonic Generation Microscopy for Human Brain Imaging
Sandeep Chakraborty;Hui-Yuan Chen;Ru-Pin Chi;Chen-Tung Yen;Hsin-Yi Hunag;Chi-Kuang Sun

In this study, we demonstrated high-resolution label free human brain imaging using second and third-harmonic generation microscopy.Fixed human brain tissues were imaged. Second harmonic generation images can clearly distinguish the plaques while the third harmonic generation images show neuronal processes as well as lipid-rich myelin sheaths in the white-matter.

09:45 - 10:00 Paper No.  2017-FRI-S0603-O006
Yuan-Ron Lo Background Cancellation of Temporal Focusing-based Multiphoton Excited Fluorescence Images via Fast Structured Illumination
Guan-Wei Chen;Yuan-Ron Lo;Yvonne Yuling Hu;Feng-Jie Lai;Chih-Long Chang;Shean-Jen Chen

We have simulated temporal focusing-based multiphoton excited fluorescence image, and then use two-dimensional patterns in structured illumination image and uniform illumination image to be reconstructed by single sideband demodulation based HiLo, local variation based HiLo, and Hilbert transform based HiLo to get superior optically sectioned images.

10:00 - 10:15 Paper No.  2017-FRI-S0603-O007
Cian You Integration of OCT with Raman spectroscopy for skin cancer detection
Cian You;Jheng Yu Wu;Wan Lin Jiang;Ming Yi Lin;Jeng Wei Tjiu;Sheng Lung Huang

The OCT tomogram can guide the position precisely to obtain specific Raman spectra. As a demonstration, the melanoma cell line and biopsied tissue were measured. With the Raman shift from amide III (1321.1 cm-1), methylene (1470.6 cm-1), and amide I (1661.6 cm-1), the melanoma cell line can clearly be identified.