Session Index

Biophotonics and Biomedical Imaging

Poster Session I
Thursday, Dec. 7, 2017  12:30-14:00
Presider: -
Room: International Research Building 5F
Paper No.  2017-THU-P0601-P001
Yao-Chung Chan Award Candidate Wavelength Tunable Light Source with multi-modulation frequencies for frequency-domain bio-photonic applications
Yao-Chung Chan;Ming-Mao Lee;Sheng-Hao Tseng;Ming-Che Chan

In this work, we present a novel scheme of a simple, fast (MHz to GHz), intensity-modulated light source of various wavelengths with high modulation depth in the 0.7~1.35 micrometers bio-penetration window. A frequency-domain diffuse optical spectroscopy was utilized as a demonstration example.

Paper No.  2017-THU-P0601-P002
Cheng Chia-Yi Award Candidate Microstructural changes in leaf aging at epidermis layer observed by high resolution Full Field Optical Coherence Microscopy (FF-OCM)
Cheng Chia-Yi;Anna Tulsi;Chakraborty Sandeep;Chiou Arthur;Kuo Wen-Chuan

We report a high resolution FF-OCM with a supercontinuum light source to investigate the microstructural changes in epidermis layer of maple leaves which showed significant deterioration in red leaves (aged) compared to green leaves. Our approach can be useful in developing hand-held FF-OCM system for quality maintenance in agricultural field.

Paper No.  2017-THU-P0601-P003
Ting-Li Lin Award Candidate Sensitivity Enhancement of Functionalized MoS2 Based Plasmonic Biosensors for Detection of Pregnancy Associated Plasma Protein A2 (PAPP-A2)
Ting-Li Lin;Nan-Fu Chiu

The development of carboxyl-functionalized molybdenum disulfide (MoS2-COOH) has led to a new trend in the biosensing field. In summary, the excellent binding affinity, biocompatible and high sensitivity suggest the potential of the clinical application of this MoS2-COOH-based SPR chip detection method for in vitro diagnostic and point-of-care testing devices.

Paper No.  2017-THU-P0601-P004
Chun Ling Yen Award Candidate Label free of hybridized asymmetric metamaterial resonator with high Q factor
Chun Ling Yen

A resonance-based sensing device for biomedical applications which is operation at 30 THz. When the biological cells are attached to the device surface, its resonance frequency will shift, making possible calibration-free, speedy biological cell detection.Focus on the devices as a refractive index detector with high-Q to improve the resolution.

Paper No.  2017-THU-P0601-P005
Chuan-Hsiang Yu Award Candidate Cost-Effective Wireless Multichannel NIRS Embedded System
Chuan-Hsiang Yu;Chia-Wei Sun

Near-infrared spectroscopy (NIRS) is a popular technique for therapeutic applications in recent years. However, most of the commercial NIRS systems are expensive, and it limits the prevalence. We built an inexpensive, wearing-convenient, light in weight, and rechargeable NIRS system. In this presentation we test our system with mental algorithm task.

Paper No.  2017-THU-P0601-P006
Yung-Chun Tsai Award Candidate Sensitivity Enhancement Of Functional Single Layer MOS2 Based Surface Plasmon Resonance Biosensor For Detection Of CA19-9.
Yung-Chun Tsai;Ting-Li Lin;Nan-Fu Chiu

In this experiment, we present a fast and stable process carboxyl modified molybdenum disulfide (MoS2-COOH) as an ultra-sensitive surface plasmon resonance (SPR) sensing layer. The characteristic of high sensitive and biological affinity MoS2-COOH bio-chip is a novel and advantage technique.

Paper No.  2017-THU-P0601-P007
Han Ko Award Candidate Investigating the iron oxide nanoparticles on selective wavelength photothermal therapy for antibacterial activity
Han Ko;Chih-Chia Huang

We demonstrate a high photothermal conversion efficiency iron oxide nanoparticles with extending absorption spectrum from UV to NIR. In environment area, we present the solar driven photothermal antibacterial activity. For therapeutic application, we choose the high tissue transmittance 808nm NIR laser to prove the inhibition of microbial infection.

Paper No.  2017-THU-P0601-P008
Yung-Ching Chang Award Candidate Dual-function Tags For Photothermal Therapy and Raman Imaging of Glioblastoma Cells
Yung-Ching Chang;Li-Ching Huang;Yi-Syuan Wu;Chun-I Sze;Shiuan-Yeh Chen

In this work, dual-function tags were developed to apply on photothermal therapy and Raman imaging of GBM (Glioblastoma Multiforme), a fatal brain tumor. Due to the LSP-enhanced photothermal conversion efficiency and SERS effect of core-satellite gold nanoparticles, the tags have the abilities to kill and specifically image GBM cells.

Paper No.  2017-THU-P0601-P009
Meng-Syuan Jian Award Candidate Dynamic Phase Analysis on Spectral Interferometry-based Surface Plasmon Resonance Biosensing
Meng-Syuan Jian;Meng-Huan Tsai;Chung-Hsuan Chen;Chou-Yun Hsu;Shih-Hsiang Hsu

A spectral interferometry-based surface plasma resonator system demonstrates the Influenza DNA sensitivity as 0.149 μg/mL. Its phase related association and dissociation constants obtained from characterization are 9759.72 (s-1 M-1) and 0.0438 (s-1), respectively. To our knowledge, it is the first time to derive the dynamic phase analysis in telecommunication wavelengths.

Paper No.  2017-THU-P0601-P010
Tien-Yu Hsiao Award Candidate Intraoperative Optical Imaging for Brain Tumor Surgery
Tien-Yu Hsiao;Xin-Rui Liu;Kai-Shih Chiu;Yun-Qian Li;Chun-Jung Huang;Shan-Ji Li;Ching-Po Lin;Chia-Wei Sun

Accurate surgeries hold the key to good prognoses of brain tumors. An intraoperative optical intrinsic signal imaging is employed to distinguish lesions. The result shows vessels in tumor region have poor ability to provide oxygen.

Paper No.  2017-THU-P0601-P011
pan yi Award Candidate Implementation of a three-dimensional tracking system with harmonic generation microscopy diagnosis for multiple facial actinic keratoses
pan yi

We establish a high definition face tracking system with three-dimensional coordinate mapping and apply this 3D face tracking system to determine the location and treatment efficacy of multiple facial AKs in clinical follow-ups. Finally, we investigate the clinical applicability of HGM imaging for diagnosis of AK in vivo.

Paper No.  2017-THU-P0601-P012
Mei Ru Chen Award Candidate Differentiation of glioma and white matter using polarization-sensitive optical coherence tomography
Mei Ru Chen

In this study, we classified birefringent and non-birefringent tissues by statistical methods. Since tumor is birefringent tissue and white matter is not, we successfully drew the boundary between tumor and white matter using PS-OCT. Our goal is to provide precisely maximal resection for operators.

Paper No.  2017-THU-P0601-P013
Pei Yu Lai Award Candidate Angiography and Lymphangiography for Study of Skin Melanoma Mice Using Optical Coherence Tomography
Pei Yu Lai;Tim-Han Lin;Ya-Shuan Chou;Chung-Hsing Chang;Wen-Chuan Kuo

We have developed a multi-contrast, label free optical coherence tomography (OCT) imaging system, which can provide the information of microstructure and microcirculatory system simultaneously. Using this technique, we observed angiogenesis, lymphangiogenesis and structural information in the ear of the skin melanoma mice in vivo.


Biophotonics and Biomedical Imaging

Imaging & Microscopy I
Thursday, Dec. 7, 2017  13:00-14:45
Presider: Erh-Te Chiu
Room: International Research Building IR5008(5F)
13:00 - 13:30 Paper No.  2017-THU-S0601-I001
Invited Speaker:
Tomoyuki Yokota
Ultra-flexible organic photonic devices
Tomoyuki Yokota

Recently, we have fabricated 1-µm-thick ultra-flexible and lightweight organic electronics, such as organic solar cell, organic polymer light-emitting diodes and organic transistor. These electronics exhibit extraordinarily tough mechanical robustness such as minimum bending radius of 5 μm. By integrating ultra-flexible green and red PLEDs with an OPD, a flexible and conformable reflective pulse oximetry has been demonstrated. In order to detect pulse waves and blood oxygen levels, the device was turned over and wrapped around a finger. While the driving voltage of the PLEDs was set at 5 V, the open-circuit voltage (Voc) of the OPD was monitored for measuring the absorption of green and red light in the blood to the pulse.

13:30 - 13:45 Paper No.  2017-THU-S0601-O001
Jih-Liang Hsieh Award Candidate Temporal Focusing-based Multiphoton Excitation Microscope with Temporal Spectral Compensation
Jih-Liang Hsieh;Chia-Yuan Chang;Chun-Yu Lin;Shean-Jen Chen

Temporal spectral dispersions of ultrashort pulses degrade both the excitation efficiency and the axial resolution in temporal focusing-based multiphoton excitation microscopy. A pulse dispersion measurement utilizing spectral interferometry and a frequency component phase delay modulation utilizing a deformable mirror are embedded into a field-programmable gate array to compensate the dispersions.

13:45 - 14:00 Paper No.  2017-THU-S0601-O002
Yu-Kai Chao Award Candidate Simultaneous multiphoton imaging of calcium-ion dynamics and sarcomere contraction on single primary cardiomyocytes isolated from transgenic zebrafish
Yu-Kai Chao;Yu-Ching CHANG;Ian LIAU

To study the excitation-contraction coupling of contracting myocardium, two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) were employed to image simultaneously calcium-ion dynamics and sarcomere contraction of single primary cardiomyocytes, which was isolated from a transgenic line of zebrafish that expresses fluorescent proteins sensitive to intracellular concentration of calcium-ion.

14:00 - 14:15 Paper No.  2017-THU-S0601-O003
Yu-Tung Chen Award Candidate An image comparison of in-vivo rat cornea between full-field optical coherence tomography and confocal reflectance microscopy
Yu-Tung Chen;Ting-Wei Hsu;Ting Cheng;Wei-Li Chen;Rajendran Soundararajan;Sheng-Lung Huang

Using the full-field optical coherence tomography on in-vivo rat cornea, both lateral and axial images were obtained with sub-micron resolutions. When compared with the confocal reflectance microscope, the layered structure of cornea can be resolved by the FF-OCT. In addition, the nucleus of the superficial cell can clearly be seen.

14:15 - 14:30 Paper No.  2017-THU-S0601-O004
Hsu Chia-Wei Award Candidate Rapid volumetric multiphoton imaging with the combination of an ultrasound lens and a resonant mirror
Hsu Chia-Wei;Chiang Tsung-Yen;Lin Chun-Yu;Chang Chia-Yuan;Chang Chih-Long;Chen Shean-Jen

The combination of a tunable acoustic gradient (TAG) lens and a resonant mirror were integrated into a multiphoton excited (MPE) microscope that is rapidly and precisely controlled by an embedded field programmable gate array (FPGA) to acquire images at high frame rate.

14:30 - 14:45 Paper No.  2017-THU-S0601-O005
Jia-Pu Syu Award Candidate Multi-contrast Ultrahigh Resolution Optical Coherence Tomography for Rat Retina imaging.
Jia-Pu Syu;Min-Jyun Su;Po-Wei Chen;Chang-Chih Ke;Wen-Chuan Kuo;Shih-Hwa Chiou

This study presents a polarization sensitive optical coherence tomography angiography for in vivo high resolution imaging of retina in SD rat. Combining these contrasts, this platform will be useful for the quantitative evaluation of retinal degeneration, treatment after therapy, and drug screening development in the future.


Biophotonics and Biomedical Imaging

Biosensing and Detection
Thursday, Dec. 7, 2017  15:15-17:00
Presider: Chia-Wei Sun;Chao-Sheng Wang
Room: International Research Building IR5008(5F)
15:15 - 15:30 Paper No.  2017-THU-S0602-O001
I-Chieh Chen Award Candidate Attention Evaluation in Different Lighting Environments by Using EEG and Empirical Mode Decomposition
I-Chieh Chen;Chin-Hsien Wu;Yi-Chun Chen

This study aims to evaluate users’ attention levels under different lighting environments by using brainwaves. The empirical mode decomposition followed by Fourier transforms is used to analyze the signals. After that, we utilize the improved power spectra and band-power histograms to compare the brainwaves by binary discrimination tasks.

15:30 - 15:45 Paper No.  2017-THU-S0602-O002
Chien-Nan Chen Award Candidate Development of see-through binocular synchronous pupilometer
Chien-Nan Chen;Yi-Chun Tasi;Yung-Jhe Yan;Mei-Lan Ko;Ni-Chung Lee;Mang Ou-Yang

This research brings out a non-invasive, see-through binocular synchronous pupilometer to detect ANS. From the related research, the neurotransmission could be observed the response of the human’s pupils. We develop two pupilometers synchronously with a deviation less than 1/60 second to record the response of two pupils.

15:45 - 16:00 Paper No.  2017-THU-S0602-O003
Bo-Shun Jaing Award Candidate Electrical Detection of Streptavidin-Biotin Complex Using IGZO Thin Film Transistors Integrated with Microfluidic Channels
Bo-Shun Jaing;Jian-Jang Huang;Nian-Ting Wu

We demonstrate an IGZO-TFT-based biosensor integrated with a Y-type external microfluidic channels for the detection of the mixing reaction situation of the Streptavidin and Biotin. By observing the real-time current change of the TFT biosensor, the biochemical reaction can be analyzed.

16:00 - 16:15 Paper No.  2017-THU-S0602-O004
Chien-Chun Chen Award Candidate Photonic Crystal Biosensor for Glucose Detection
Chien-Chun Chen;Yu-Yang Liao;Yung-Tsan Chen;Jian-Jang Huang

Here we demonstrate a photonic crystal (PhC) sensor. The PhCs are able to diffract optical beams to various angles in the space. The critical wavelength that satisfies the phase matching or becomes evanescent is used to analyze the target. In the glucose detection, our sensor demonstrates high sensitivity.

16:15 - 16:30 Paper No.  2017-THU-S0602-O005
Hsiung Chan-Te Award Candidate Real-time, Unprocessed, whole blood sensing using microfluidic chip incorporated with guided-mode resonance biosensor
Hsiung Chan-Te;Tsai Meng-Zhe;Chen Yang;Huang Cheng-Sheng;Hsu Hsin-Yun;Hsieh Pei-Ying

We demonstrate the detection of C-creative protein from whole blood by using a system consisting of a microfluidic chip and a guided-mode resonance biosensor. The chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the GMR biosensor for real-time monitoring.

16:30 - 16:45 Paper No.  2017-THU-S0602-O006
Yu-Zi Lin Award Candidate Isotropic Quantitative Differential Phase Contrast Microscopy
Yu-Zi Lin;Hsi-Hsun Chen;Kuang-Yuh Huang;Yuan Luo

To improve the accuracy and stability of phase recovery in conventional DPC effectively, we propose a new illumination method to achieve isotropic differential phase contrast (iDPC) efficiently. Our iDPC is implemented with a partially coherent microscopy, and a TFT shield to modulate the illumination pattern.

16:45 - 17:00 Paper No.  2017-THU-S0602-O007
Tzu Hsin Fan Detecting hard tissue in vivo by near-infrared
Tzu Hsin Fan

We use the new measurement of bone mineral density (BMD) to predict osteoporosis. Its performance is tested on 10 subjects by nears infrared (NIR) image system. Comparing with the dual energy x-ray absorptiometry (DXA) and get the excellent correlation coefficient.


Biophotonics and Biomedical Imaging

Poster Session II
Thursday, Dec. 7, 2017  15:30-17:00
Presider: -
Room: International Research Building 5F
Paper No.  2017-THU-P0602-P001
Yun-Ting Huang Fabrication of Gelatin Tissue Phantoms Containing Bilirubin to Simulate Jaundice Skin
Yun-Ting Huang;Sheng-Hao Tseng;Yi-Ling Lin

The condition of Jaundice can be non-invasively quantified with a Diffuse Reflectance Spectroscopy (DRS) system. To characterize the system performance, it is essential to fabricate gelatin phantoms containing various bilirubin concentrations. We will reveal phantoms’ fabrication processes and the effect of the detection probe’s contact force on the measurement results.

Paper No.  2017-THU-P0602-P002
Wei-Li Chien Quantified age-related difference in hemodynamic response during WCST by NIRS
Wei-Li Chien;Che-Wei Chen;Chia-Wei Sun

The present study elucidates the quantified results reflect the time-varying trends of hemodynamic response during the neuropsychological test, the Wisconsin Card Sorting Test (WCST). We utilized the real-time near-infrared spectroscopy (NIRS) and WCST performance to understand the age effects on cerebral hemodynamics.

Paper No.  2017-THU-P0602-P003
HUSAN-HAO HUANG High resolution optical measurement system used in optical glucose measurement
HUSAN-HAO HUANG;Chin-Jung Chuang

Diabetes is an important disease and typically requires the person with the disease to monitor their blood sugar level via finger or forearm sticks multiple times daily.The system used the match template to solve the non-invasive optical measurement limit on Resolution.

Paper No.  2017-THU-P0602-P004
Min-Chun Pan Design and Implementation of PMT Calibration Module for Optical Imaging System
Pei-Jyun Shih;Jhao-Ming Yu;Min-Chun Pan;Min-Cheng Pan;Liang-Yu Chen;Yan-Yang Shu

Based on the photoelectric correction module designed in this study, the signal acquisition platform and calibration program of the photo-detector are establish, which can obtain the characteristic curve of the PMT accurately-and-quickly. The method is applied to the DOT system to improve the accuracy of the system measurement results.

Paper No.  2017-THU-P0602-P005
Hung-Chun Wang Non-Axial Line-Scanning Multifocal Confocal Microscopy
Hung-Chun Wang;Chou-Min Chia;Yu-Hsin Chia;Yuan Luo

Confocal microscopy has been widely used to acquire optional sectioning fluorescent image. However, conventional confocal microscopy requires time-consuming scanning in depth. In this article, we experimentally demonstrate a non-axial depth scanning method for multi-plane imaging with no depth scanning by incorporating multiplexed volume holographic gratings (MVHGs) with slit aperture.

Paper No.  2017-THU-P0602-P006
Po-Shyang Yang Establishment of Non-invasive Blood Pressure System by Using PPG
Po-Shyang Yang

Photoplethysmography can get variation of blood volume on fingertip and lots of physiological information. Based on PPG photoelectric technology and arterial hemodynamics, we present a new physiological parameter, ΔRI(Delta Reflection Index). In this paper, we compare the new method with PWV method, then get better effect.

Paper No.  2017-THU-P0602-P007
Yin-Quan Chen Cancer Cells Invasion in Cancer Spheroid Model Studied via Time-Lapse Microscopy
Yin-Quan Chen;Jean-Cheng Kuo;Ming-Tzo Wei;Yen-Chih Chen;Muh-Hwa Yang;Arthur Chiou

We investigated the correlation between the invasion capability of OECM-1 spheroids and the surrounding collagen deformation via traction force microscopy and time-lapse microscopy. We observed that highly-invasive OECM-1 spheroids generated a high magnitude of collagen deformation at the very early stage.

Paper No.  2017-THU-P0602-P008
Ting-Pei Chang System Calibration and Imaging Model Construction of Single Photon Emission Computed Tomographic System
Bo-Yung Huang;Ting-Pei Chang;Yi-Chun Chen

A system calibration method is developed for single photon emission computed tomographic systems. The procedure includes the geometric calibration, grid-scan experiment and imaging model construction. The system matrix required in the tomographic reconstruction is then generated accordingly. The results of phantom imaging validates the proposed calibration procedure.


Biophotonics and Biomedical Imaging

Imaging & Microscopy II
Friday, Dec. 8, 2017  08:30-10:15
Presider: Yi-Chun Chen;Chia-Wei Sun
Room: International Research Building IR5008(5F)
08:30 - 08:45 Paper No.  2017-FRI-S0603-O001
Sung-Liang Chen Miniature Scan Head for All-Optical Optical-Resolution Photoacoustic Microscopy
Zhendong Guo;Guangyao Li;Sung-Liang Chen

Optical-resolution photoacoustic microscopy (OR-PAM) provides high lateral resolution and has demonstrated broad biomedical applications. We demonstrate a miniature scan head for all-optical OR-PAM using gradient-index lenses and a Fabry-Perot optical ultrasound detector. The scan head is easy to build and can be further developed for endoscopic and intravascular applications.

08:45 - 09:00 Paper No.  2017-FRI-S0603-O002
Ting-Wei Hsu Spectroscopic Full-Field Optical Coherence Tomography on In Vivo Rat Cornea Analysis
Ting-Wei Hsu;Yu-Tung Chen;Sheng-Lung Huang

Spectroscopic full-field optical coherence tomography (S-FFOCT) for in-vivo biomedical samples is demonstrated. Such optical system enabled high speed tomographic imaging as well as the spectroscopic analysis of the scanned data. Depth-dependent spectral peak shifts are observed in in-vivo rat cornea analysis.

09:00 - 09:15 Paper No.  2017-FRI-S0603-O003
Jye-Chang Lee The identification of tumor margin for extramammary Paget’s disease with harmonic generation microscopy
Jye-Chang Lee;Yi-Hua Liao;Chi-Kuang Sun

Identifying the tumor border of extramammary Paget’s disease (EMPD) by visual inspection is not accurate. Although skin biopsy provides a gold-standard of diagnosis and surgical margins, it’s invasive and time-consuming. Here, we presented the applications of HGM on determining the margins of EMPD, which is consistent with the histopathological results.

09:15 - 09:30 Paper No.  2017-FRI-S0603-O004
Feng Chun Hsu Volumetric Bioimaging Based on Light Field Microscopy with Temporal Focusing Illumination
Feng Chun Hsu;Yong Da Sie;Chia Yuan Chang;Feng Jie Lai;Shean Jen Chen

Light field technique at a single shot can get the whole volume image of observed sample. By integrating a light field microscopy to a temporal focusing-based multiphoton excitation fluorescence microscope, the excitation volume can be controlled. Therefore, light field microscope based on temporal focusing obtains three-dimensional image with less noise.

09:30 - 09:45 Paper No.  2017-FRI-S0603-O005
Sandeep Chakraborty Label-free Second and Third Harmonic Generation Microscopy for Human Brain Imaging
Sandeep Chakraborty;Hui-Yuan Chen;Ru-Pin Chi;Chen-Tung Yen;Hsin-Yi Hunag;Chi-Kuang Sun

In this study, we demonstrated high-resolution label free human brain imaging using second and third-harmonic generation microscopy.Fixed human brain tissues were imaged. Second harmonic generation images can clearly distinguish the plaques while the third harmonic generation images show neuronal processes as well as lipid-rich myelin sheaths in the white-matter.

09:45 - 10:00 Paper No.  2017-FRI-S0603-O006
Yuan-Ron Lo Background Cancellation of Temporal Focusing-based Multiphoton Excited Fluorescence Images via Fast Structured Illumination
Guan-Wei Chen;Yuan-Ron Lo;Yvonne Yuling Hu;Feng-Jie Lai;Chih-Long Chang;Shean-Jen Chen

We have simulated temporal focusing-based multiphoton excited fluorescence image, and then use two-dimensional patterns in structured illumination image and uniform illumination image to be reconstructed by single sideband demodulation based HiLo, local variation based HiLo, and Hilbert transform based HiLo to get superior optically sectioned images.

10:00 - 10:15 Paper No.  2017-FRI-S0603-O007
Cian You Integration of OCT with Raman spectroscopy for skin cancer detection
Cian You;Jheng Yu Wu;Wan Lin Jiang;Ming Yi Lin;Jeng Wei Tjiu;Sheng Lung Huang

The OCT tomogram can guide the position precisely to obtain specific Raman spectra. As a demonstration, the melanoma cell line and biopsied tissue were measured. With the Raman shift from amide III (1321.1 cm-1), methylene (1470.6 cm-1), and amide I (1661.6 cm-1), the melanoma cell line can clearly be identified.


Biophotonics and Biomedical Imaging

Poster Session III
Friday, Dec. 8, 2017  12:30-14:00
Presider: -
Room: International Research Building 5F
Paper No.  2017-FRI-P0603-P001
Ching-Hsiang Chan Using off-specular microscopic imaging ellipsometry for biosensing aided by 1D reflection grating
Ching-Hsiang Chan;Yu-Da Chen;Ming-Lin You;Pei-Kuen Wei;Yia-Chung Chang

We performed spectroscopic ellipsometry and microscopic imaging ellipsometry measurements of the off-specular reflection signals of objects on various reflection gratings. We show that spectral ellipsometry in off-specular reflection mode can be used as an effective method for sensitive optical detection of foreign objects, which is especially useful for biosensing.

Paper No.  2017-FRI-P0603-P002
Sunil Vyas Multiplane Edge Contrast Enhancement Imaging Using Forked Shaped Defocus Grating
Sunil Vyas;Chen Yen Lin;Yuan Luo

We report on the implementation of spiral phase contrast imaging at multiple planes using forked-shaped defocus grating. The dual function of grating helps in simultaneous generation of multiple edge enhanced images corresponding to different depths. Present method is simple, direct and is applicable to coherent and incoherent imaging system.

Paper No.  2017-FRI-P0603-P003
KA KUN WU Photoacoustic Image Reconstruction Based On Scalar Diffraction Theory
KA KUN WU;An Di Tang;Te-Yuan Chung

According by the scalar diffraction theory calculation and structure correction calculation, we rebuild the double point photoacoustic image, which include the signal’s amplitude and phase information.

Paper No.  2017-FRI-P0603-P004
Chen Jia Xuan Automated Identification of Low Dose Computed Tomography Subtle Nodules
Chen Jia Xuan

In this study, we designed a computer-aided diagnostic system. Calculate the characteristic parameters of the nodule image and put them into the inverted neural network to determine the results. The accuracy is 75% with true positive 75% and true negative 75%.

Paper No.  2017-FRI-P0603-P005
Wei-Han Hung Differentiation of esophageal cancer cells by multiphoton fluorescence lifetime imaging microscopy (M-FLIM)
Wei-Han Hung;I-Chen Wu;Wei-Chung Chen;Ming-Tsang Wu;Chie-Tong Kuo;Fu-Jen Kao;Guan-Yu Zhuo

Esophageal cancer has fatally attacked human health over the past few decades. Here we present a novel quantitative analytical tool that can be used to discriminate among the various types of esophageal cancers prior to diagnosis. Our cancer cell classification results were confirmed using multiphoton fluorescence lifetime imaging microscopy.

Paper No.  2017-FRI-P0603-P006
Yu-Cheng Lin Design of LMR Based Sensor with ITO-Coated POF
Yu-Cheng Lin;Shang-chen Wei;Wen-xiao Luo;Yongjie Xu;Shuting Xu;Bo-yu Lin

The design of plastic optical fiber sensor based on lossy-mode resonance was studied. The sensing device is a D-shaped plastic optical fiber and a thin-film layer of ITO is deposited. The influence of the thickness of optical fiber and ITO on the sensitivity is evaluated theoretically.

Paper No.  2017-FRI-P0603-P007
YI-JU LO Hydrogen peroxide concentration measurement by polarization spectral analysis technique
YI-JU LO;Te-Yu Chiang;Yu-Jen Chen;Cheng-Chih Hsu

This study demonstrated the H2O2 concentration measurement with polarization spectrometry. Based on the experiment results, the absorbance of the product is related to the H2O2 concentration and polarized state of the incident light. The best sensitivity and resolution can be reached 1478 absorbance/% and 1.3510-5 %, respectively.


Biophotonics and Biomedical Imaging

Biomedical Related Optics and Photonics I
Friday, Dec. 8, 2017  13:00-14:30
Presider: Han-Sheng Chuang;Po-Chen Hu
Room: International Research Building IR5008(5F)
13:00 - 13:15 Paper No.  2017-FRI-S0604-O001
Ging-rue Liu Opto and magneto dual imaging platform for rapid ex-vivo and in-vivo discrimination of tumors
Ging-rue Liu;Wen-Chun Wei;Jen-Jie Chieh

A novel dual-imaging model system integrating an optical camera and magnetic scanning superconducting-quantum-interference device (SQUID) biosusceptometry (SSB) was proposed. The simultaneous coregistration of low-field magnetic images of MNP distributions and optical images of anatomical regions enabled the tumor distribution to be determined easily and in real time.

13:15 - 13:30 Paper No.  2017-FRI-S0604-O002
CHANG FU-KAI Ultra-compact illumination system design for dual view capsule endoscope
CHANG FU-KAI;Sheu Ming-Jen;Pan Jui-Wen

We use two prescribed illumination distributions to compensate for the low relative illumination. In the optical simulation, we obtain an illumination uniformity of 67.86% and an efficiency of 33.45% for the front view area and an illumination uniformity of 74.92% and an efficiency of 19.32% for the back view area.

13:30 - 13:45 Paper No.  2017-FRI-S0604-O003
Tulsi Anna Optical Visualization and Morphological Analysis of Leaves using Spectral Domain Optical Coherence Tomography (SD-OCT)
Tulsi Anna;Sandeep Chakraborty;Chia-Yi Cheng;Arthur Chiou;Wen Chuan Kuo

Here, a non-destructive spectral domain optical coherence tomography (SD-OCT) system to monitor morphological changes in autumn phenology (senescence) of Acer serrulatum leaves was reported. Structural changes in the epidermis and thickness variation in palisade layer has been observed.This system has the potential to generate quality control in agricultural field.

13:45 - 14:00 Paper No.  2017-FRI-S0604-O004
Shi-Xuan Yang PSTD simulation of expending beam width with the presence of a scattering medium
Shi-Xuan Yang;Snow H. Tseng

We employ the pseudospectral time-domain (PSTD) simulation technique to model light distribution through the scattering media. To analyze the scattering characteristics of light, various factors are analyzed. Specifically, scattering medium consisting of diverse scatter sizes resulting in a different angular span of out-coming light.

14:00 - 14:15 Paper No.  2017-FRI-S0604-O005
Wen-Chun Wei Noninvasive, Biochemical Consumable-Free, and Fast Examination of Orchid Diseases
Wen-Chun Wei;Yen-Hsiang Wang;Kuan-Chieh Lee;Ming-Hsien Chiang;Shien-Kuei Liao;Jen-Jie Chieh

The spectrum analysis for the noninvasive examination of orchid virus is a challenge due to time-varied biological conditions of live individuals. In this work, two algorithm analysis were proposed to discriminate orchid diseases. The sensitivities are 77.5 % and 88 % for the calibration analysis and Gaussian decomposition analysis, separately.

14:15 - 14:30 Paper No.  2017-FRI-S0604-O006
Han Lee Here we model an acoustic wave source propagating out of needle by employing finite-difference time-domain simulation technique. To analyze phenomenon, various factors are analyzed, including wavelength of acoustic wave, speed of sound, density of medium and size of needle. Finally, simulation results are used to help analyze experimental measurements
Han Lee;Snow H. Tseng

Here we model an acoustic wave source propagating out of needle by employing the finite-difference time-domain simulation technique. To analyze phenomenon, various factors are analyzed, including wavelength of acoustic wave, speed of sound, density of medium and size of needle. Finally, simulation results are used to help analyze experimental measurements


Biophotonics and Biomedical Imaging

Poster Session IV
Friday, Dec. 8, 2017  14:30-16:00
Presider: -
Room: International Research Building 5F
Paper No.  2017-FRI-P0604-P001
Zhe-Xun Zhuang The study of optical properties and preparation of MoS2 nanoparticles
Zhe-Xun Zhuang;Yun-Chung Chou;Hsiang-Chen Wang

MoS2 powder was prepared into MoS2 nanoparticle dispersion in NMP solvent by ultrasonic method, and the optical properties were measured by optical measurement. Then we observed the size and the morphology of the MoS2 nanoparticles by EDX, SEM and TEM. And their material properties by Raman measurement.

Paper No.  2017-FRI-P0604-P002
Yueh-Ling Hsu Development of dual optical imaging with SERS-nanocore@fluorescent BSA for cellular tracing and monitoring in targeting delivery
Yueh-Ling Hsu;Chih-Chia Huang

Integration of fluorescent Au NCs@BSA and SERS-based AuAg-4NTP are presented to approach the dual optical applications in cellular imaging. Modifying with CPBA and RGD, the internalization in cancer cells could be enhanced and thus improved the optical signals. The fluorescence-combined SERS provided complementary imaging analysis for tracking tissue and cells.

Paper No.  2017-FRI-P0604-P003
Liang-Chieh Huang Kinetics of Photoprotection Pathways Resolved by FPGA-Based Fluorescence Lifetime System
Pei-Xuan Lan;Liang-Chieh Huang;Yeh Lin;Yi-Chun Chen

We constructed a multi-wavelength fluorescence lifetime measurement system based on Field Programmable Gate Array. With this system, we changed the wavelength of illumination light in the fluorescence lifetime experiments, and we observed the kinetic characteristics of two different photoprotection pathways in photosynthessis systems in vivo.

Paper No.  2017-FRI-P0604-P004
Liang-Yu Chen The Application of Analytical Solution of Diffusion Equation to Verify Diffuse Optical Imaging System
Liang-Yu Chen;Ya-Ting Liang;Jhao-Ming Yu;Min-Cheng Pan;Min-Chun Pan

Diffuse optical imaging can reconstruct distribution of optical properties in tissue for breast cancer detection based on diffusion equation. In this study, analytical solution of diffusion equation is applied for verifying imaging system by comparing the solution and measurement data.

Paper No.  2017-FRI-P0604-P005
Quoc-Hung Phan Differential Mueller Matrix Formalism for Glucose Sensing with High Scattering Effects
Jian-Xiang Lin;Yu-Lung Lo;Quoc-Hung Phan

Differential Mueller matrix formalism is proposed for glucose sensing with high scattering effects. The feasibility of the proposed technique is evaluated using aqueous phantom solution with concentrations ranging from 0-400 mg/dL. The practical applicability of the proposed technique is demonstrated by measuring the depolarization index of human fingertip tissue.

Paper No.  2017-FRI-P0604-P006
Yu-Chian Lin Automatic Iris Multispectral Imagery with Machine Learning
Yu-Chian Lin;Chun-Hao Chu;Jian-Jia Su;Chung-Hao Tien

In this study, we developed an automatic system to retrieve the spectral image of an iris. Compared with the ground truth iridal spectra by the spectrometer measurement, proposed digital scheme can restore the spectra with error_rms about 0.012 in randomly selected six subjects.

Paper No.  2017-FRI-P0604-P007
Shuai-Yan Chen Dual-color interferometric light scattering microscopy implemented in optofluidic channels
Shuai-Yan Chen;Guan-Yu Zhuo

Using dual-wavelength illumination for high-speed single-particle tracking not only offers the information of diffusion behavior of nanoparticles but also the scattering spectral properties at particle surfaces. In this study, metallic nanoparticles transported through optofluidic channels under different environmental conditions are investigated and analyzed by interferometric light scattering microscopy.

Paper No.  2017-FRI-P0604-P008
Jau Sheng Wang Photonic nanojets simulate by Zemax for laser surgery
Jau Sheng Wang

Focusing of light has been used for microprobes which is stable and good-confined beam of photons is scanned or directed over area of a biological sample or structure. Like focusing microprobes may be useful for laser surgery, optical endoscopy and spectroscopy and photo-induced patterning of thin films.


Biophotonics and Biomedical Imaging

Biomedical Related Optics and Photonics II
Friday, Dec. 8, 2017  14:50-16:20
Presider: Yi-Chun Chen;Han-Sheng Chuang
Room: International Research Building IR5008(5F)
14:50 - 15:05 Paper No.  2017-FRI-S0605-O001
Yi-Lun Chiang Protein quantitation based on optical absorption of dye-doped liquid crystal
Yi-Lun Chiang;Mon-Juan Lee;Wei Lee

We developed a biosensing method using a dye-doped liquid crystal. The working system was demonstrated with a black dichroic dye possessing high dichroic ratio for promoting the sensitivity in detecting biomolecules. A polarizer used to make incident light linearly polarized enhanced the resolving power of the detection system.

15:05 - 15:20 Paper No.  2017-FRI-S0605-O002
Ching-Min Lin Protein detection and quantitation based on dielectric responses of dual-frequency liquid crystal
Ching-Min Lin;Po-Chang Wu;Mon-Juan Lee;Wei Lee

This research exploited dielectric properties of dual-frequency liquid crystal (DFLC) to demonstrate its capability of quantitative protein detection. The proposed DFLC-based biosensing can eliminate experimental errors caused by variation in cell gap. Linear correlation between the protein concentration and dielectric responses at designated frequencies was obtained by dielectric spectroscopy.

15:20 - 15:35 Paper No.  2017-FRI-S0605-O003
Kuang-Li Lee Tailoring the Evanescent Field Decay Lengths of Surface Plasmon Resonances Using Alumina-Coated Aluminum Nanoslits
Kuang-Li Lee;Po-Cheng Tsai;Meng-Lin You;Ming-Yang Pan;Pei-Kuen Wei

We propose the combination of capped aluminum nanoslits and a thin nanodielectric top layer to improve the surface sensitivities of nanostructure-based aluminum sensors. We find that the passivation layer can enhance the wavelength sensitivities of the asymmetric Fano resonance in capped aluminum nanoslits by a factor of 3.5.

15:35 - 15:50 Paper No.  2017-FRI-S0605-O004
Yung-Tsan Chen Photonic Crystal Biosensor for Epstein–Barr Virus Protein Detection
Yung-Tsan Chen;Yu-Yang Liao;Jian-Jang Huang

A 2-D photonic crystal biosensor with cost-effective measurement system was developed in this study. By analyzing the spectra of the diffraction beam,and using Epstein–Barr nuclear antigen-1 (EBNA-1) antibody as analyte, the biosensor is able to detect 10 ng/mL of EBNA-1 antibody in the phosphate buffered saline (PBS) solution.

15:50 - 16:05 Paper No.  2017-FRI-S0605-O005
Ting-Ying Li Development of a neurofeedback training system based on wearable NIRS device to improve attentional capability in children with Tourette syndrome
Ting-Ying Li;Pou-Leng Cheong;Chia-Wei Sun

NIRS neurofeedback training serves as a physical therapy for Tourette syndrome children not only to decrease the drug side effect but to improve SNR in comparison to EEG. We built a neurofeedback system including a training software and a wearable NIRS device with 4 channel covering prefrontal area.

16:05 - 16:20 Paper No.  2017-FRI-S0605-O006
Jia-Hao Li Simulation analysis of the transmittance of Cornea and Sclera
Jia-Hao Li;Snow H. Tseng

Cornea and Sclera both consist of collagen fibrils, but the optical characteristics aren’t the same. Using the pseudospectral time-domain (PSTD) simulation technique, changing different sizes and arrangements of the non-absorbing collagen fibrils and analyzing the scattering characteristics of light of the cornea and sclera.