Session Index

Biophotonics and Biomedical Imaging

Imaging & Microscopy I
Thursday, Dec. 7, 2017  13:00-14:45
Presider: Erh-Te Chiu
Room: International Research Building IR5008(5F)
13:00 - 13:30 Paper No.  2017-THU-S0601-I001
Invited Speaker:
Tomoyuki Yokota
Ultra-flexible organic photonic devices
Tomoyuki Yokota

Recently, we have fabricated 1-µm-thick ultra-flexible and lightweight organic electronics, such as organic solar cell, organic polymer light-emitting diodes and organic transistor. These electronics exhibit extraordinarily tough mechanical robustness such as minimum bending radius of 5 μm. By integrating ultra-flexible green and red PLEDs with an OPD, a flexible and conformable reflective pulse oximetry has been demonstrated. In order to detect pulse waves and blood oxygen levels, the device was turned over and wrapped around a finger. While the driving voltage of the PLEDs was set at 5 V, the open-circuit voltage (Voc) of the OPD was monitored for measuring the absorption of green and red light in the blood to the pulse.

13:30 - 13:45 Paper No.  2017-THU-S0601-O001
Jih-Liang Hsieh Award Candidate Temporal Focusing-based Multiphoton Excitation Microscope with Temporal Spectral Compensation
Jih-Liang Hsieh;Chia-Yuan Chang;Chun-Yu Lin;Shean-Jen Chen

Temporal spectral dispersions of ultrashort pulses degrade both the excitation efficiency and the axial resolution in temporal focusing-based multiphoton excitation microscopy. A pulse dispersion measurement utilizing spectral interferometry and a frequency component phase delay modulation utilizing a deformable mirror are embedded into a field-programmable gate array to compensate the dispersions.

13:45 - 14:00 Paper No.  2017-THU-S0601-O002
Yu-Kai Chao Award Candidate Simultaneous multiphoton imaging of calcium-ion dynamics and sarcomere contraction on single primary cardiomyocytes isolated from transgenic zebrafish
Yu-Kai Chao;Yu-Ching CHANG;Ian LIAU

To study the excitation-contraction coupling of contracting myocardium, two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) were employed to image simultaneously calcium-ion dynamics and sarcomere contraction of single primary cardiomyocytes, which was isolated from a transgenic line of zebrafish that expresses fluorescent proteins sensitive to intracellular concentration of calcium-ion.

14:00 - 14:15 Paper No.  2017-THU-S0601-O003
Yu-Tung Chen Award Candidate An image comparison of in-vivo rat cornea between full-field optical coherence tomography and confocal reflectance microscopy
Yu-Tung Chen;Ting-Wei Hsu;Ting Cheng;Wei-Li Chen;Rajendran Soundararajan;Sheng-Lung Huang

Using the full-field optical coherence tomography on in-vivo rat cornea, both lateral and axial images were obtained with sub-micron resolutions. When compared with the confocal reflectance microscope, the layered structure of cornea can be resolved by the FF-OCT. In addition, the nucleus of the superficial cell can clearly be seen.

14:15 - 14:30 Paper No.  2017-THU-S0601-O004
Hsu Chia-Wei Award Candidate Rapid volumetric multiphoton imaging with the combination of an ultrasound lens and a resonant mirror
Hsu Chia-Wei;Chiang Tsung-Yen;Lin Chun-Yu;Chang Chia-Yuan;Chang Chih-Long;Chen Shean-Jen

The combination of a tunable acoustic gradient (TAG) lens and a resonant mirror were integrated into a multiphoton excited (MPE) microscope that is rapidly and precisely controlled by an embedded field programmable gate array (FPGA) to acquire images at high frame rate.

14:30 - 14:45 Paper No.  2017-THU-S0601-O005
Jia-Pu Syu Award Candidate Multi-contrast Ultrahigh Resolution Optical Coherence Tomography for Rat Retina imaging.
Jia-Pu Syu;Min-Jyun Su;Po-Wei Chen;Chang-Chih Ke;Wen-Chuan Kuo;Shih-Hwa Chiou

This study presents a polarization sensitive optical coherence tomography angiography for in vivo high resolution imaging of retina in SD rat. Combining these contrasts, this platform will be useful for the quantitative evaluation of retinal degeneration, treatment after therapy, and drug screening development in the future.